Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: The oncofetal protein glypican-3 is a novel marker of hepatic progenitor/oval cells.

doi: 10.1038/labinvest.3700479

Figure Lengend Snippet: Figure 5 mRNA expression analysis of isolated Thy1 positive progenitor and PDGFR-b positive stellate cells. Thy1 þ cells were isolated from 2-AAF/PH liver at day 10 after surgery and PDGFR- b þ cells were isolated from D-gal-treated liver. (a) RT-PCR analysis of AFP, CD45, CK-19, GAPDH, Gpc3 and Thy1 mRNA expression in FACS sorted Thy1 positive/CD45 negative progenitor cells from 2-AAF/PH liver. (b) RT-PCR analysis of AFP, CD45, CK-19, desmin, GAPDH, and Gpc3 mRNA expression in PDGFR-b positive stellate cells isolated with microbeads from D-gal-treated liver.

Article Snippet: For cell sorting monoclonal anti-rat CD45 PE (cat num CL009PE) and anti-rat Thy1 (CD90) FITC (cat num CL005F) were purchased from Cedarlane (Burlington, NC, USA) and used at dilution 1:50.

Techniques: Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction

Journal: The Journal of Experimental Medicine

Article Title: Treatment with Soluble Interleukin-15Rα Exacerbates Intracellular Parasitic Infection by Blocking the Development of Memory CD8 + T Cell Response

doi: 10.1084/jem.20011915

Figure Lengend Snippet: The effect of sIL-15R treatment on the proliferation of adoptively transferred memory CD8 + T cells. Congenic Thy1.1 mice ( n = 5) were infected orally with 10–15 cysts of T. gondii and CD44 hi CD8 + T cells were isolated on day 14 and transferred intravenously (10 6 /mouse) to naive Thy1.2 animals. 2 wk after the transfer, the recipient animals ( n = 4) were challenged with 80 cysts of T. gondii and treated with T1 or M4 as described in . The animals were given BrdU via drinking water (0.8 mg/ml) for 7 d. Recipients were killed on day 8 and spleen and liver were analyzed for BrdU + Thy1.1 + T cells by flow cytometry. Data are mean ± SD ( n = 4). *, P < 0.01; **, P < 0.001.

Article Snippet: After washing in FCM, the spleen and liver cell preparations (2.5 × 10 5 /well) were labeled with PE-labeled anti-Thy1.1 antibody in 96-well round-bottom polypropylene microtiter plates (Costar Corp.), incubated for 30 min on ice, and then washed twice with 200-μl changes of FCM buffer.

Techniques: Infection, Isolation, Flow Cytometry